| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Passive Samplers, a Powerful Tool to Detect Viruses and Bacteria in Marine Coastal Areas
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| Autores: |
Vincent Hubert, Francoise
Wacrenier, Candice
Morga, Benjamin
Lozach, Solen
Quenot, Emmanuelle
Mege, Mickael
Lecadet, Cyrielle
Gourmelon, Michele
Hervio-heath, Dominique
Le Guyader, Soizick
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| Data: |
2021-02
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| Ano: |
2021
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| Palavras-chave: |
Norovirus
Ostreid herpes virus 1 μ
Var
Vibrio spp
Microbial source tracking
Sea
Passive sampler
Oyster (Crassostrea gigas)
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| Resumo: |
The detection of viruses and bacteria which can pose a threat either to shellfish health or shellfish consumers remains difficult. The current detection methods rely on point sampling of water, a method that gives a snapshot of the microorganisms present at the time of sampling. In order to obtain better representativeness of the presence of these microorganisms over time, we have developed passive sampling using the adsorption capacities of polymer membranes. Our objectives here were to assess the feasibility of this methodology for field detection. Different types of membrane were deployed in coastal waters over 2 years and the microorganisms tested using qPCR were: human norovirus (NoV) genogroups (G)I and II, sapovirus, Vibrio spp. and the species Vibrio alginolyticus, V. cholerae, V. vulnificus, and V. parahaemolyticus, OsHV-1 virus, and bacterial markers of fecal contamination. NoV GII, Vibrio spp., and the AllBac general Bacteroidales marker were quantified on the three types of membrane. NoV GII and OsHV-1 viruses followed a seasonal distribution. All membranes were favorable for NoV GII detection, while Zetapor was more adapted for OsHV-1 detection. Nylon was more adapted for detection of Vibrio spp. and the AllBac marker. The quantities of NoV GII, AllBac, and Vibrio spp. recovered on membranes increased with the duration of exposure. This first application of passive sampling in seawater is particularly promising in terms of an early warning system for the prevention of contamination in oyster farming areas and to improve our knowledge on the timing and frequency of disease occurence.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00682/79375/81893.pdf
https://archimer.ifremer.fr/doc/00682/79375/81894.docx
https://archimer.ifremer.fr/doc/00682/79375/81895.docx
https://archimer.ifremer.fr/doc/00682/79375/81896.docx
https://archimer.ifremer.fr/doc/00682/79375/81897.docx
https://archimer.ifremer.fr/doc/00682/79375/81898.docx
https://archimer.ifremer.fr/doc/00682/79375/81899.docx
https://archimer.ifremer.fr/doc/00682/79375/81900.docx
https://archimer.ifremer.fr/doc/00682/79375/81901.docx
DOI:10.3389/fmicb.2021.631174
https://archimer.ifremer.fr/doc/00682/79375/
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| Editor: |
Frontiers Media SA
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| Relação: |
info:eu-repo/grantAgreement/EC/FP7/678589/EU//VIVALDI
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| Formato: |
application/pdf
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| Fonte: |
Frontiers In Microbiology (1664-302X) (Frontiers Media SA), 2021-02 , Vol. 12 , P. 631174 (12p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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